**The title, authors, and abstract for this completion report are provided below.  For a copy of the completion report, please contact the GLFC via e-mail or via telephone at 734-662-3209**

DEVELOPING A LAMPREY CONTRACEPTIVE

Michael E. Baker

Department of Medicine, University of California, San Diego

9500 Gilman Drive, La Jolla, CA 92093-0693

May 2011

ABSTRACT:

We tested a series of chemicals identified by using Autodock 4 to screen a chemical database at the

National Cancer Institute (NCI]) for selective inhibition of lamprey estrogen receptor (ER) compared

to human ERα and ERβ. The goal was to find specific inhibitors of lamprey ER that could be used

for controling reproduction in lamprey females. None of the original set of eight chemicals that were

found using Autodock 4 inhibited estradiol activation of lamprey ER, human ERα and human ERβ in

a transcriptional activation assay using HEK293 cells. We also tested two additional chemicals that

were identified from the NCI diversity set using Vina, another docking algorithm. These two

chemicals also did not inhibit activation by estradiol of lamprey ER human ERα and ERβ as

determined in a transcriptional activation assay using HEK293 cells. We are continuing to search

other databases for potential selective inhibitors of lamprey ER.